Figure 1

Schematic flowchart of the experimental settings and research strategy applied in this study. For the control treatment (CK), tobacco plants were exposed to cold stress for 6 h; for cold acclimation (CA) treatment, tobacco plants were first transferred to 15 °C conditions for 6 h and then exposed to cold stress. Physiological data were collected at 0 h, 6 h and 12 h, and RNA-seq and metabolomics data were collected after 0 h and 6 h of CA treatment.