Figure 3
Stochastic DNA methylation changes on both the WT and KO alleles after passage of FBN1 het KO fibroblast cells. (a) RM allele ratio of the FBN1 het KO fibroblast cells (n = 6) at P6 and P20. In the S region, sequenced reads with ≥75% unmethylated CpG sites (i.e. ≥7 out of nine CpGs) were defined as RM alleles. Based on the sodium bisulfite sequencing data in Fig. 2, RM allele ratios were calculated for the WT and KO alleles, respectively, and are shown as average ± SD. Statistical comparisons of the RM allele ratios were performed using the Student’s t-test, and statistical significance was set as p < 0.05. (b) Changes in RM allele ratios of FBN1 het KO fibroblast cells from P6 to P20. Solid and dotted lines indicate the RM allele ratio of WT and KO alleles, respectively. (c) Summary of changes in RM allele ratios and FBN1 mRNA levels in FBN1 het KO fibroblast cells. The data of the FBN1 mRNA is presented in Fig. 1. Black and white triangles indicate the increase and decrease in RM allele ratios and FBN1 mRNA levels from P6 to P20, respectively. (d) Correlation between FBN1 mRNA level and RM allele ratio. The difference in FBN1 mRNA level and RM allele ratio between P6 and P20. Closed circles represent the data of each FBN1 het KO fibroblast cell (n = 6).
