Figure 5
DNA methylation status of FBN1 CpG island shore and FBN1 expression in human cells. The DNA methylation status of five human somatic cell lines (MRC5, AM936EP, Edom22, PAE551, and UtE1104) (a) and twelve human iPS cell lines (b) were analyzed with the Infinium Human Methylation450K BeadChip. In the human FBN1 upstream region, three probes (probe I, II, and III) that are located in the outside region (probe I), the CpG island shore (probe II), and inside the CpG island (probe III) of the human FBN1 locus. DNA methylation levels are presented as a pie chart; black and white areas indicate the percentages of methylated and unmethylated CpGs, respectively. (c) The correlation between FBN1 mRNA level and DNA methylation level of FBN1 CpG island shore (probe II) was calculated for human iPS cell lines (n = 12). FBN1 expression and DNA methylation data were based on the gene expression array and the Infinium Human Methylation450K BeadChip, respectively.
