Figure 4 | Scientific Reports

Figure 4

From: Evidence that ERF transcriptional regulators serve as possible key molecules for natural variation in defense against herbivores in tall goldenrod

Figure 4

Involvement of jasmonate and ethylene signaling in transcriptional regulation of SaERF. (a) Leaves were pretreated with aqueous solution (mock), salicylhydroxamic acid (SHAM) or silver thiosulfate (STS). Transcript accumulation levels of SaERF1 and SaERF2 in undamaged S1 and S2 leaves and leaves 120 min and 30 min after mechanical damage (MD), respectively, were determined. Data represent the mean ± standard error (n = 4–5). Data marked with an asterisk are significantly different from those of mock treatment, based on an ANOVA with Holm’s sequential Bonferroni post-hoc test (**, 0.001 ≤ P < 0.01; *, 0.01 ≤ P < 0.05). Otherwise, the mean followed by a P-value is marginally different. (b) Transcript accumulation levels of SaERF1 and SaERF2 in response to exogenous application of methyl jasmonate (MeJA) solution (0.5 mM) or ethylene (ET) gas (1 ppm) for 120 min. Relative transcript abundances were determined after normalization of raw signals with the abundance of the housekeeping transcript of a histone gene (CL2599.Contig7). Data represent the mean ± standard error (n = 4–5). Data marked with an asterisk are significantly different based on an ANOVA from the respective of mock treatment (*, 0.01 ≤ P < 0.05). ns, not significant.

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