Figure 6

Defense property of SaERF-expressing Arabidopsis lines. (a) Transcript levels of defensin gene PDF1.2 in the leaves of wild-type (WT) plants, SaERF1-expressing plants (ERF1-OX1 and ERF1-OX2), SaERF2-expressing plants (ERF2-OX2 and ERF2-OX3) and vector control (VC) plants. Relative transcript abundances were determined after normalization of raw signals with the abundance of the housekeeping transcript of the Arabidopsis ACT8 gene (At1g49240). Data represent the mean and standard error (n = 5). (b) The net body weight that Spodoptera litura larvae gained during 2 days or 4 days after they had been placed on potted plants of WT or transgenic lines (VC or SaERF-expressing plants [ERF1-OX1, ERF2-OX2, ERF-OX2, and ERF-OX3]). Data represent the mean and standard error (n = 12–15). Data marked with an asterisk are significantly different from those of WT, based on an ANOVA with Holm’s sequential Bonferroni post-hoc test (**, 0.001 ≤ P < 0.01; *, 0.01 ≤ P < 0.05).