Figure 4

Daily running wheel activity (A), body weight (B), and body temperature (C), during a two weeks ad libitum (AL) phase, and a food restricted (RF) phase of 7 days of duration. Rats were treated with leptin (open symbols, ○, □) or vehicle (closed symbols, ●, ▲, ■) via implanted minipumps beginning on the first day of RF phase. Ambient temperature (Ta) during RF was maintained at 21 °C (solid line) or 25 °C (dot dash line), as in AL phase, or increased to 32 °C (dotted line). Vertical dashed line separates Al y RF phases. ^**p < 0.01 and ^***p < 0.001 for 21 °C vs 25 °C. ^###p < 0.001 for 21 °C vs 32 °C. ^$p < 0.05 and ^$$$p < 0.001 for 25 °C vs 32 °C. ^&p < 0.05 for 21VH vs 21LEP. ^!!!p < 0.001 for 21VH vs 32VH and 21LEP vs 32VH. Results expressed as mean ± SEM.