Figure 2 | Scientific Reports

Figure 2

From: Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses

Figure 2

Protective capacities of selected strains in a mouse model of C. rodentium infection. Individual bacterial strains Lr 5454, and Bl 5764 (5 × 108 CFU of each) were daily administered 5 days prior to infection until the day of sacrifice to C57BL/6J mice (n = 10 mice per group), while control infected or non-infected (control) mice received only PBS. Mice were orally inoculated with 1 × 109 CFU C. rodentium at day 0, sacrificed 9 days after infection and compared to non-infected control mice. (A) Bacterial load was assessed in the feces over the course of the experiment. (B) Detection of lipocalin-2 in feces by ELISA at different time, (C) at day 5 and (D) at day 7. (E) Correlation of lipocalin-2 with bacterial load at day 5. (F) Colon length, (G) Crypt length (May-Grünwald and Giemsa stained histology samples) and (H–J) gene expression of inflammatory markers (Il1b, Il6 and Tnfa) in the proximal colon were evaluated after the sacrifice at day 9. Results are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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