Figure 1 | Scientific Reports

Figure 1

From: Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK

Figure 1

CNK2 is a membrane-associated protein and localised in the post-synapse of neurons. (A) CNK2 is expressed from P0 throughout adulthood in the mouse brain. Whole cell lysates generated from mouse brains at P0, P10, P20 and adult stage were analysed by SDS-PAGE and western blot with anti-CNK2 antibody. Mortalin serves as loading control (expected size 60 kDa). (B) Membrane localisation of overexpressed EGFP-tagged CNK2 (EGFP-CNK2) in CHL V79 cells (EGFP-CNK2 cyan; DAPI blue). Scale bar: 20 µm. (C) Localisation of endogenous CNK2 (cyan) and the dendritic marker MAP2 (magenta) in cultured rat hippocampal neurons (DIV21). Endogenous CNK2 is localised in punctate structures along the dendrite. Scale bar: 20 µm (D) Localisation of EGFP-CNK2 (cyan) following lentiviral transduction, and the dendritic marker MAP2 (magenta) in cultured rat hippocampal neurons (DIV21). Scale bar: 20 µm. (E) CNK2 (cyan) colocalises with postsynaptic marker PSD-95 (magenta). (F) CNK2 (cyan) shows adjacent staining with the presynaptic marker Synapsin (magenta). Scale bar: 10 µm.

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