Figure 2 | Scientific Reports

Figure 2

From: Disease-associated synaptic scaffold protein CNK2 modulates PSD size and influences localisation of the regulatory kinase TNIK

Figure 2

Loss of CNK2 reduces PSD size. (A) Cultured rat hippocampal neuron infected with lentivirus transducing shRNA stained for the postsynaptic marker Homer (magenta) and dendritic marker MAP2 (cyan). Scale bar: 10 µm. (B) Sample image of endogenous Homer thresholded for analysis of Homer particle size. (C) PSD size, represented by the signal of the postsynaptic marker protein Homer (arbitrary units), was analysed using the “Analyze Particles” tool (FIJI/Image). Data were normalised to the median of the control. Graph represents the mean ± SEM (n = 7–10 neurons, N = 3 cultures): control (scrambled shRNA) = 1 ± 0.03, CNK2 knock down = 0.81 ± 0.06; Mann-Whitney test, p < 0.0097. (D) Spine density upon lentiviral transduction of scramled control or CNK2 knock down. Homer positive spines were counted. Graph represents the mean of number of spines/100 µm ± SEM (n = 10–13 neurons, N = 4 cultures): control (scrambled shRNA) = 60 ± 7, CNK2 knock down = 66 ± 9; unpaired t-test, p = 0.6501.

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