Figure 3 | Scientific Reports

Figure 3

From: miR-216a-targeting theranostic nanoparticles promote proliferation of insulin-secreting cells in type 1 diabetes animal model

Figure 3

Verification of miR-216 and PTEN expressions in pancreatic islets performed on consecutive sections from NOD mice. All experiments were performed in triplicate. (A) In situ hybridization analysis of miR-216a expression in pancreatic islets from 3-, 8- and 18-week old NOD mice (bottom, miR-216a: green, magnification bar = 50 μm); Insulin (red) and CD3 (green) immunostaining of consecutive sections (top, cell nucleus – blue). (B) Immunostaining of PTEN (green) and CD3 (red) expression in pancreatic islets from 3-, 8- and 18-week old NOD mice (bottom, cell nucleus - blue, magnification bar = 50 μm); Immunostaining of Ki67 (green) and CD3 (red) expression in pancreatic islets from 3-, 8- and 18-week old NOD mice (top, cell nucleus – blue). Note Ki67 positive cells located in the periphery of the damaged islets of 3 and 8 wks old mice (yellow arrows). In contrast, in 18 wks old mice Ki67 positive cells (red arrow) were located in the central area of the damaged islet, where the residual insulin producing cells are located on the consecutive section in (A).

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