Figure 3
Non-motor deficits characterize the early SCA1 stage in mice better than does ataxia. (a) Latency on the accelerating rotarod over 5 days (D) of experimentation. The points are means ± SEMs. The P-values are based on comparing the averaged values from the whole experiment via the permutational t-test. See Suppl. Tables S9–S11 for the detailed results. (b) Visualization of the dissimilarities in the gait parameters using non-metric multidimensional scaling (NMDS). The P-values are based on the permutational multivariate analysis of variance (PERMANOVA). The ellipses show the 90% confidence intervals for point occurrence (each point = 1 animal). (c,d) ROC curve and area under the ROC curve showing the ability of the sensitive indicators to correctly classify the mice into genotypes. The vertical lines imply 95% confidence intervals. The significances are based on bootstrapping and indicate the difference between the AUC of a given indicator versus the AUC of rotarod latency. *P < 0.05, **P < 0.01, ***P < 0.001. n.s. = not significant. w.a. = age in weeks. OF = open field. MWM = Morris water maze. FST = forced swimming test. See Methods for numbers of animals per each experimental group.
