Figure 5

Impaired hippocampal neurogenesis in the SCA1 mice. (a) Representative images of hippocampal sections stained for NeuN. Scale bars (a,b) = 300 µm. (b) Representative images of the DG double stained for DCX and PSA-NCAM (with DAPI). (c) NeuN immunofluorescence intensity in the CA1 (left) and CA2/3 (middle-left) pyramidal layers and the density of NeuN+ neurons (per 1,000 µm²) in the same hippocampal regions (right). (d) Density of DCX⁺PSA-NCAM⁺ neurons per 100 µm of the DG subgranular zone. (e) Density of DCX⁺ dendrites crossing the line on the border of the DG-G and DG-ML (M/G; left), inner part of the DG-ML (M-in; middle) and outer part of the DG-ML (M-out; right), per 100 µm. (f) PSA-NCAM immunofluorescence in the DG polymorph layer (left; DG-P), DG-ML (middle-left), CA4 pyramidal (middle-right; CA4-P) and CA1 stratum lacunosum-moleculare (right; CA1) hippocampal layers. N (c-f) = 5 animals per group (with 4 sections per animal). Each point = 1 section. The statistical significances were based on the permutational linear mixed-effect models (See Suppl. Tables S25 and S26 for the detailed results) using 4 replicates per animal and with the animal identity representing the random-effect factor. (g) Hippocampal BDNF level (pg/mg of proteins). N = 6 animals per group. P = 0.046 (permutational t-test). Each point = 1 animal. Box-whisker plots (c–g) indicating the inter-quartile (IQ) intervals (box), 1.5*IQ range (whiskers) and medians (middle line). *P < 0.05, **P < 0.01, ***P < 0.001. n.s. = not significant.