Figure 3

The ratio of avocadene and avocadyne dictates SEDDS droplet size and stability. (A) Effect of 3:1 avocadene-avocadyne concentration on average hydrodynamic diameter (Z-average) of NeoBee—Tween 80 SEDDS over time. Inset: visual appearance of control (blank SEDDs) and 3:1 avocadene-avocadyne containing SEDDS on day 0. (B) Polydispersity index of SEDDS described in (A). (C) Effect of 3:2 avocadene-avocadyne concentration on Z-average of NeoBee—Tween 80 SEDDS over time. Inset: visual appearance of control (blank SEDDs) and 3:2 avocadene-avocadyne containing SEDDs on day 0. (D) Polydispersity index of SEDDs described in (C). For A–D, values are means ± SEM of three independent experiments. Note: size and polydispersity data for both 3:1 and 3:2 avocadene-avocadyne at a concentration of 20 mg/mL are missing on day 3 and onwards due to emulsion destabilization.