Figure 6
Avocado polyols exert less toxicity in non-AML cell lines when delivered in either DMSO or SEDDS. In vitro cytotoxicity of avocado polyols was evaluated in non-AML cell lines (Caco-2, INS-1 (832/13), C2C12 myotubes and HepG2). Cell lines were incubated with varying concentrations of avocado polyols dissolved in DMSO or as SEDDS. After 24 hours, cell viability was measured by the MTS assay. For avocadyne (A), avocadene (B), avocatin B (C) and 3:1 avocadene-avocadyne (D), 20 mg/mL (2% w/w) SEDDS were freshly prepared and used. For 3:2 avocadene-avocadyne (E), 15 mg/mL (1.5% w/w) SEDDS were freshly prepared and used. Data fit to nonlinear regression curve as described for Fig. 5. All data represents mean ± SEM from three independent experiments performed in triplicate.
