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Figure 1

From: Infrared Spectroscopic Imaging Visualizes a Prognostic Extracellular Matrix-Related Signature in Breast Cancer

Figure 1

Summary of methods. In comparing IR imaging to gene expression profiling, we took a multi-step approach. Imaging was followed by an automated analysis of spectral data for each pixel using machine learning. From the assignment of cellular and acellular identity of all pixels in the image, collagen dominant pixels were analyzed. The spectral properties of these pixels were related to a label (ECM3, non-ECM3) for further analysis.

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