Figure 3 | Scientific Reports

Figure 3

From: Hypoxia-inducible factor-1 alpha maintains mouse articular cartilage through suppression of NF-κB signaling

Figure 3

Regulation of cartilage degradation by HIF-1α. (A) Amount of glycosaminoglycans released into the medium determined by the dimethylmethylene blue assay during 3 days culture of Hif1afl/fl (Cntl) and Col2a1-CreERT2;Hif1afl/fl (KO) femoral heads treated with or without 10 ng/mL IL-1β under the hypoxic condition (3% O2). Tamoxifen induction was performed three days before sacrifice. Bars show the mean ± SD of three samples per group. *P < 0.05. (B) Immunofluorescence of Mmp13, Hif2a, and Hif1a in the Cntl and KO femoral head cartilage after 3-day culture with or without 10 ng/mL IL-1β. Scale bars, 50 µm.

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