Figure 1 | Scientific Reports

Figure 1

From: A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties

Figure 1

The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

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