Table 3 Results of pathogenicity tests. Chi-squared tests were used to estimate the differences in proportions, and t-tests were used to estimate the differences between means.

From: Occurrence and pathogenicity of Corinectria spp. – an emerging canker disease of Abies sibirica in Central Siberia

Parameter

Isolate N1 (NfP5.7)

Isolate N2 (NfSp3.4)

Pooled N1 + N2

Control

Saplings of Abies sibirica

Tested (no.)

30

30

60

30

Dead & diseased (no./%)

– dead

7/23.3

5/16.7a

12/20.0

2/6.7c

– diseased, phloem necrotic

20/66.7

21/70.0a

41/68.3

0/0d

Visually healthy (no./%)

3/10.0

4/13.3a

7/11.7

28/93.3d

Phloem necrosis (mm, mean ± SD)

– longitudinal

46.9 ± 3.0

41.9 ± 2.5b

44.4 ± 2.8

0 ± 0d

– lateral at cross-section

18.2 ± 0.8

15.9 ± 0.6b

17.1 ± 0.7

0 ± 0d

Re-isolation of the fungus (no./%)

24/80.0

22/73.3

46/76.7

—

Seeds and seedlings of Abies sibirica

Tested (no.)

300

300

600

300

Germinated seeds (no./%)

199/66.3

203/67.7a

402/67.0

210/70.0c

Dead & diseased (no./% of germinated)

169/84.9

165/81.3a

334/83.1

18/8.6d

Chip coverage by mycelium (no. chips checked/mean score e)

45/2.2

45/1.9

90/2.1

—

Re-isolation of the fungus from

– chips (no. checked/no. isolated/%)

24/22/91.7

24/20/83.3

48/42/87.5

—

– inoculated seedlings (no./%)

156/92.3

147/89.1

303/90.7

—

Seeds and seedlings of Picea abies

Tested (no.)

300

300

600

300

Germinated seeds (no./%)

118/39.3

125/41.7a

243/40.5

234/78.0d

Dead & diseased seedlings (no./% of germinated)

84/71.2

86/68.8a

170/70.0

0/0d

Seedling length (mm, mean ± SD)

– stem

24.4 ± 2.0

28.1 ± 2.2b

26.3 ± 1.4

41.0 ± 2.4d

– main root

4.7 ± 0.4

6.7 ± 0.5b

5.7 ± 0.4

18.9 ± 1.4d

whole

29.1 ± 4.6

34.8 ± 3.0b

32.0 ± 2.6

59.9 ± 8.6d

Seedling dry weight (mg, mean ± SD)

0.60 ± 0.1

0.65 ± 0.03a

0.61 ± 0.03

1.9 ± 0.1d

Re-isolation of the fungus (no./%)

80/95.2

79/91.9

159/93.6

—

  1. aDifference between the tested isolates (N1 vs. N2) non-significant.
  2. bDifference between the tested isolates (N1 vs. N2) significant at p < 0.05.
  3. cDifference between both tested isolates and control (pooled N1 + N2 vs. control) non-significant.
  4. dDifference between both tested isolates and control (pooled N1 + N2 vs. control) significant at p < 0.0001.
  5. eEvaluated visually, scoring system: score 4: mycelium covers surface of an inoculated chip by >75%; score 3: 50–75%; score 2: 25–50%; score 1: <25%; score 0: mycelium absent.
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