Figure 2
Higher variability of mitochondrial mass in FECD explants. (A) A marker of mitochondrial mass (mitotracker) was used in healthy and FECD endothelial explants. The typical CEC loss and the enlargement of remaining CECs in the FECD explants (right) compared to the healthy explants (left) is well illustrated. (B) Quantification of the mitotracker signal showed no statistically significant difference in mitochondrial mass between healthy and FECD explants. However, FECD cells presented more variation (Coefficient of variation (CV) = 0.44) than the Healthy cells (CV = 0.17). Scale bar = 40 μm. Median and standard deviation are reported. Experiments were performed with 3 different explants from Healthy (1200 cells analyzed, N = 3) and 3 FECD patients (1176 cells analyzed, N = 3).
