Table 4 Overlapping PCR-primer pairs used for amplification of different segments of the OsPLDα1.

From: Novel allelic variation in the Phospholipase D alpha1 gene (OsPLDα1) of wild Oryza species implies to its low expression in rice bran

Primer ID

Forward Primer (5′ to 3′)

Reverse Primer (5′ to 3′)

Amplicon sizea

PLD-1

TTTAACCTCGCCTCCTCC

TCTCCAATTCTTGTCTACTACC

783 bases (−154–629)

PLD-2

GCCCGAATTTGATCTGCT

TTTGGAATGAAGTTGTCTGG

946 bases (545–1472)

PLD-3

GGAGAGGAGATTGACAGATGG

AGGAGAAGGTGGAATAATAGTG

995 bases (1259–2233)

PLD-4

CATGATATTCACTCACGGCT

TGTAACTCATCTGACATGCT

862 bases (2114–2957)

PLD-5

CTACCTCACTTTCTTCTGCT

ATGTCCCAGTACTTCTCC

885 bases (2749–+16)

  1. aNumbers in parenthesis shows the position of bases covered by the primer pairs on OsPLDα1 locus.
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