Figure 2
From: Inhibition of HDAC6 activity protects dopaminergic neurons from alpha-synuclein toxicity
Proteomic analysis of vehicle- and Tubastatin A-treated alpha-synuclein-injected rats. (A) Brain region including substantia nigra (purple circle) of three vehicle-treated (aSyn + Veh) and three Tubastatin A-treated (aSyn + TubA) alpha-synuclein-injected rats were used to perform label-free quantitative proteomic analysis. (B) Hierarchical clustering heatmap of Pearson correlation coefficients between all animals used in this experiment. The highest correlations are obtained between animals within the same group (vehicle or Tubastatin A). (C) Principle component analysis (blue: vehicle; red: Tubastatin A). (D) Volcano plot of differentially regulated proteins in response to Tubastatin A treatment in alpha-synuclein injected rats. Proteins were graphed by fold change (difference) and significance (-Log p) using a false discovery rate of 0.01 and an SO of 0.1. Significantly up-regulated proteins were shown as red dots and down-regulated proteins were shown as blue dots using the Perseus software. (E) Each down- or up-regulated protein from the Tubastatin A group was assigned a unique label that best represented its function. The number of proteins within a specific function is shown.
