Figure 4
From: Inhibition of HDAC6 activity protects dopaminergic neurons from alpha-synuclein toxicity
Effect of tubastatin A on the acetylation of alpha-synuclein. (A,B) Representative immunofluorescence confocal microscopy images of the substantia nigra area (purple square on brain representation) showing staining for acetylated lysines (Ac-lysines) (red), human alpha-synuclein (h-aSyn) (green) and DAPI (blue) at low magnification (A) and at high magnification (B), and quantification of fluorescence using integrated density of acetylated lysines (acK)- and alpha-synuclein–positive cells (**P < 0.01; n = 20 cells/rat/group in all rats) (C) in the alpha-synuclein-injected side of rats treated with vehicle (Veh) or Tubastatin A (TubA). White squared numbers (B) show representative co-stained cells used for quantification. (C) Scale bar = low magnification, 100 µm; high magnification, 20 μm. White squares on the top panel show the localization of the high magnification area. White arrows point on co-localized double staining cells.
