Figure 5
From: Inhibition of HDAC6 activity protects dopaminergic neurons from alpha-synuclein toxicity
Tubastatin A reduces astrocyte reactivity in the substantia nigra of alpha-synuclein-injected rats. (A,C) Representative immunofluorescence confocal microscopy images of the substantia nigra area showing staining for Gfap (A), S100β (C) and DAPI in the contralateral (Cont) and ipsilateral (Ipsi) sides of alpha-synuclein-injected rats treated with vehicle (Veh) or Tubastatin A (TubA). Scale bar = low magnification, 100 µm; high magnification, 20 μm. (B,D) Quantification of fluorescence using integrated density of Gfap (B), S100β (D) –positive fluorescence staining intensity area in the contralateral and ipsilateral sides of the substantia nigra, expressed as percentage of the contralateral side of the alpha-synuclein-injected and vehicle-treated rats (% Cont Veh side) (*P < 0.05; **P < 0.01; ***P < 0.001; n = 7/group).
