Figure 7

Reduced expression of MCT1 transporters in RFP-TDP-43^208–414- compared with RFP-TDP-43^wt-expressing astrocytes. (A,B) Representative fluorescence images of astrocytes immunostained with antibodies against MCT1 (A) and MCT4 transporters (B, green) and labelled with DAPI (blue) in RFP-TDP-43^wt- (upper panels) and RFP-TDP-43^208–414-expressing astrocytes (lower panels; red). Immunostaining was performed 24 h after transfection with RFP-tagged TDP-43 pDNA constructs. Scale bar, 10 µm. Histograms show anti-MCT1 (A) and anti-MCT4 (B) positive cell cross-sectional area (anti-MCT1/MCT4 S; i.e. number of green fluorescence pixels with fluorescence intensity above the threshold of 20% of maximal fluorescence) per total cell cross-sectional area (cell S; i.e. number of all pixels) in RFP-TDP-43^wt- and RFP-TDP-43^208–414-expressing cells. Note the reduced expression of MCT1 transporters in RFP-TDP-43^208–414- compared with RFP-TDP-43^wt-expressing astrocytes. Numbers adjacent to the error bars depict the number of cells analysed. Data are presented as means ± SEM and acquired from four different animals. Each experiment (i.e. coverslip) was performed in duplicate, multiple cells were recorded per coverslip. **P ≤ 0.01, Mann-Whitney U test.