Figure 4 | Scientific Reports

Figure 4

From: Efficient functional screening of a cellular cDNA library to identify severe fever with thrombocytopenia syndrome virus entry factors

Figure 4

Human liver cDNA library screening using infectious virus-like particle (iVLP). (a) In screening of the human liver cDNA library to identify SFTS virus entry factors, six Jurkat colonies (Clones1–6), which consisted of Venus-expressing cells, were found on a panning dish and one of them (Clone2) is shown. (b) Propagated Clone2, control molecule (fCD2ΔCT)-expressing Jurkat cells and LSECtin-expressing Jurkat cells were examined to check their susceptibility to iVLP(Venus). (c) The genomes of naïve and Clone2 were subjected to polymerase chain reaction with a pMX primer pair. A 1-kb DNA ladder was used as a marker.

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