Figure 1
From: Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair
Genotypes of produced lambs for OTOF mutations using the CRISPR/Cas9 technology. Blue color, exon 5; Red color, exon 6; HO, homozygous; HE, heterozygous; slash (/), separates the genotypes of the two chromosomes; WT, wild-type; KO, knockout; Del, deletion; KI, insertion of STOP codons; PTC, premature termination codon due to NHEJ leading to a KO. In bold all the mutations that induce a KO. All DNA samples were extracted from skin except #63 and #66 (skin and muscle), #97 (skin, muscle and blood).
