Figure 3
From: Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair
oOTOF gene knock-out/knock-in exon 5 and 6. Panel a) Example of typical result for exon 5 PCR (left lines) and Spe I exon 5 PCR digest (right lines). Main band is around 500 bp. The bands above the main band in all animals except #73 (WT/WT) are heteroduplexes which means that NHEJ and/or KI occurred. Spe I digest revealed two bands (asterisk) under the main band of animals #74, #75 and #97 and means that a KI occurr. Panel b) Example of typical result for exon 6 PCR. Main band is around 500 bp. The bands above the main band in all animaedls except #74 and #76 (WT/WT) are heteroduplexes which means NHEJ occurred. Animal #75 shows a smaller band of ~400 bp under the main band which identifies a 198 bp deletion.
