Figure 2
From: Giant magnetoresistive biosensors for real-time quantitative detection of protease activity
(a) Design of a peptide substrate for a papain heterogeneous assay. Amino acids are listed using the standard three-letter code. (b) Standard curves for peptide loading capacity. A concentration of 2 µM was used for subsequent optical assays yielding an RFU reading of ~11,000. (c) Illustration of peptide immobilization scheme and streptavidin loading/blocking on polystyrene plates. (d) Bar graphs comparing the loading of fluorescent streptavidin Marina Blue (blue) and blocking with non-fluorescent streptavidin (orange) to immobilized peptide substrate. Error bars are ± 1σ.
