Table 1 Comparison between several protease-based assays developed and this work.
From: Giant magnetoresistive biosensors for real-time quantitative detection of protease activity
Detection principle | Protease | Buffer/Biofluid | Sensitivity | Assay time | Protease Enrichment | Sample Prep |
|---|---|---|---|---|---|---|
Colorimetric71 | Neutrophil elastase, MMPs | wound fluid | N/A | 15 min | No | No |
Colorimetric72 | Neutrophil elastase | sputum | 338 nM | 10 min | Inhibitor capture | Yes |
Colorometric78 | Trypsin | carbonate buffer | 400 pM | 3.5 hr | No | No |
Electrochemical29 | Trypsin | serum | 600 pM | 30 min | No | No |
Electrochemical30 | Trypsin | PBS, artificial serum | 20 fM | 30 min | Antibody capture | No |
Fluorescence13 | Gastricsin | Pancreatic cyst fluid | 10 nM | 1 hr | No | No |
Fluorescence79 | Prostate Specific Antigen | seminal fluid | 8 nM | 15 min | No | No |
Fluorescence62 | Proteinase 3 | sputum | 260 pM | 10 min | Inhibitor capture | Yes |
Liquid crystal33 | Trypsin, Chymotrypsin | PBS | 40 pM, 4 pM | 3 hr | No | No |
SERS32 | Trypsin | PBS | 1 nM | 5 hr | Gold nanoparticles | No |
Magnetic (this work) | Papain | PBS, urine | 4 nM, 20 nM | ~3.5 min | No | No |
