Figure 3
From: Jasmonic acid is not a biosynthetic intermediate to produce the pyrethrolone moiety in pyrethrin II
Feeding experiment using deuterium labeled (±)-MeJA. (a) Chemical structure of [2H3–12, 2H2–11, 2H1–10](±)-MeJA and pyrethrin II having expected labeled pattern. (b) UPLC MS/MS chromatogram of the leaf extract of untreated plants (control) detecting for pyrethrin II with the expected labeled pattern. (c) UPLC MS/MS chromatogram of the leaf extract of treated plants detecting for pyrethrin II having the expected labeled pattern. (d) UPLC MS/MS chromatogram of the leaf extract of treated plants detecting for endogenous pyrethrin II. Insets indicate the fragmentation patterns of deuterium-labeled pyrethrin II (i) and endogenous pyrethrin II (ii).
