Figure 2

Effect of GSTP1 I105V polymorphism on c-Jun phosphorylation and expression of proapoptotic genes in normal oral tissue in OSCC patients. (A) Representative images of an immunohistochemical analysis of adjacent normal oral tissues in OSCC patients done with anti-c-Jun phosphorylation antibody. Human samples were collected from the persons whose GSTP1 proteins having Ile/Ile or Ile/Val or Val/Val at 105 positions. (B) The level of c-Jun phosphorylation in normal oral tissues analyzed by H-score were shown. Data were analyzed by Student’s t-test. *Two-tailed p < 0.004, n = 20 for Ile/Ile and 12 for Ile/Val samples; **P < 0.0001, n = 10 for Ile/Ile and 6 for Val/Val samples. The magnification of all these images is 40X. (C,D) Effect of GSTP1 genotypes on quatitative expression of Bim and PUMA mRNA. Total RNA was isolated from tumor cells collected from samples having either GSTP1 Ile/Ile or Ile/Val or Val/Val genotypes for real-time qPCR using either Bim primers (C) or PUMA primers (D) following the procedures mentioned in the Methods. Data are plotted as a histogram. Each bar is the mean ± SD derived from 20 Ile/Ile, 12 Ile/Val and 6 Val/Val samples. The value of p < 0.05 consider to be Significant in unpaired t-test.