Figure 3 | Scientific Reports

Figure 3

From: Glutathione S-transferasesP1 AA (105Ile) allele increases oral cancer risk, interacts strongly with c-Jun Kinase and weakly detoxifies areca-nut metabolites

Figure 3

An electrostatic interactions with toxic substances at active site cavity of GSTP1 and its influence on the individual sensitivity to genotoxic effects. (A) Comparative electrostatic interactions of reduced GSH and toxic metabolites like, GA, arecoline N-oxide and arecaidenylglycine derived from raw areca-nut, and NNAL derived from tobacco, with dimeric GSTP1 proteins having Ile/Ile, Ile/Val and Val/Val at 105 positions. Red colour indicates negative charge and blue positive charge. The distance of GSH from the active pocket indicates its relative affinity for the active site residues. (B) Quantitation of 8-OHdG (ng/ml) in DNA digests was performed by ELISA-kit in both blood and tumor DNA from oral cancer patients having either RAN-chewing or RAN + tobacco-using habit. Data are plotted as a histogram. Each bar is the mean ± SD derived from 12 Ile/Ile, 12 Ile/Val and 6 Val/Val samples. The value of p < 0.05 consider to be Significant in unpaired t-test.

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