Figure 3
Salicylic acid is involved in xa5-mediated PTI. (a) Resistance of IRBB5, IR24, 24NG, B5xa5Ri and B5NG plants to Xoo T3SS mutant strain 86∆HrpXG. (b) Lesion length on leaves of rice plants at 14 dai treated with 86∆HrpXG. Values are the mean ± SD of three independent biological replicates. Letters denote significant differences based on analyses of plants treated with SA (+SA) and H2O (−SA) using the Tukey significance difference test (p < 0.05). (c) Bacterial populations of 86∆HrpXG in rice plants. Number of bacteria in 1-cm leaf sections from the cut sites were measured at 1, 4, 8 and 12 dai. Bars are means ± standard error. These plants were pretreated with 2 mM SA or H2O at the flourishing tillering stage for one day, and then inoculated with 86∆HrpXG. B5NG and 24NG are the T1 transgenic plants overexpressing the bacterial nahG gene in IRBB5 and IR24 respectively; B5xa5Ri is the xa5 RNAi plant in IRBB5 background; IRBB5V and IR24V are the transgenic plants with pCAMBIA1300 vector in IRBB5 and IR24 respectively. The scale bar is 3 cm.
