Figure 5
Effect of OsNPR3.3 in bacterial blight resistance mediated by the xa5 gene. (a,b) expression analysis of OsNPR3.3 in OsNPR3.3-overexpressing (OsNPR3.3OX) (a) and antisense-suppressing (OsNPR3.3AS) (b) T1 transgenic plants, respectively. (c) Lesion lengths of OsNPR3.3OX and OsNPR3.3AS T1 transgenic plants inoculated with PXO86. The leaves were photographed at 14 dai. (d) Phenotypes of NPR3.3 transgenic plants at the flourishing tillering stage. NPR3.3OX-4# and NPR3.3AS-3# are OsNPR3.3 overexpressing and antisense-suppressing T1 transgenic plants, respectively. (e) Expression analysis of OsNPR3.3 in IRBB5 and B5NG T1 transgenic plants inoculated with Xoo strain PXO86. Transcript levels were determined by quantitative real-time RT-PCR. Values were normalized to the expression of OsNPR3.3 in IRBB5V or IR24V, respectively. The rice actin gene served as the internal control. Bars represent the average ± SD of three biological repeats. Asterisks or pound signs indicate a statistically significant difference compared with untreated IRBB5 (*P < 0.05 and **P < 0.01). B5NG is the T1 transgenic plant overexpressing the bacterial nahG gene in IRBB5. IRBB5V and IR24V are the transgenic plants with pCAMBIA1300 vector in IRBB5 and IR24 respectively. The scale bar is 2 cm.
