Figure 2
Tomographic phase reconstructions of A. thaliana petiole fragments. 3D reconstructions of the wild type tissue (a), and the C4H mutant tissue (b). The tomograms were converted to electron density (e/Å−3), where the lower values are white and higher values are dark shades of grey. The different views are depicted by the tomogram transversal (x,y) and longitudinal (x, z) slices, highlighting the different cellular types: the vessel (V), sclerenchyma (S) and parenchyma (P) cells. Intracellular structures, such as organelles (o) in parenchymatic cells and pits (p) in vessel cells, are also indicated by the white arrows. The Fourier shell correlation (FSC) is used to estimate the spatial resolution for both reconstructed tomograms based on the half bit threshold criteria, resulting in 134 nm and 140 nm 3D resolution for the wild type and C4H mutant plant fragments, respectively.
