Figure 4

Cell wall thickness distributions of vessel and sclerenchyma cells. Analysis of the segmented vessel (a), and sclerenchyma (b), cells allowed us to observe the wall distribution and evaluate the differences in thickness between wild type (left) and C4H mutant (right) tissue fragments. The colour maps of each cell block represent the three-dimensional maps of the thickness distribution along the cell wall volume. The colour map goes from smaller thickness (violet) to larger thickness (yellow), with the values, in micrometres, highlighting the differences between wild type and mutant plants. The wall thickness analysis of each cell numbered from 1 to 3 corresponds to the histograms of thickness (µm) versus frequency.