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Figure 2

From: Sciadonic acid derived from pine nuts as a food component to reduce plasma triglycerides by inhibiting the rat hepatic Δ9-desaturase

Figure 2

The effect of Scia on the lipid profile of blood plasma. Lipids of blood plasma were extracted by Folch’s method and the FA profile was determined by GC. Lipid classes were separated by TLC and the FA from triglycerides and phospholipids were further analyzed by GC. (a) Lipid content and Δ9-desaturase indexes were determined from total lipids. (b) The TLC profiles were performed depending on the diets (20 µL of plasma per well). Triglycerides (TG) were estimated by relative quantification of the density of bands. The incorporation of Scia was determined by GC on both triglycerides and phospholipids. (c) Monoenes and Δ9-desaturase indexes were determined on the triglyceride fractions. Results on FA from TG are expressed as a % of total FA including Scia. (d) The correlation between the triglyceride concentrations in plasma and the n-7/n-9 ratios or Scia percentages of TG was determined for each diet. The significance of the difference between diets was evaluated by a t-test (*p < 0.05).

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