Table 1 Constructs, primers, plasmids and proteins.

From: Analysis of the inhibiting activity of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) on matrix metalloproteinases

Plasmid name

Protein

Parental vector(s)

Forward-primer*

Reverse-primer*

Protein sequence **

Tag***

pS6-RECK∆C

RECK∆C

pBS-hRECK

pCMV-Sport6

ATGCCCCGGGATGGCGACCGTCCGG

GCATGCGATCGCCGATGGCACACTGCTG

G27-S942 + AIA + H6

His6

pIE-RECK∆C

RECK∆C

pBS-hRECK

pIEx

TCATCGCTTTCGTCATCATCGCTGGGCCTGGCTCCGGGCAGTGCGGGTG

AAACTCAATGGTGATGGTGATGATGCGATGGCACACTGCTGGAGACCTGT

G27- S942 + H6

His6

pS6-NTES

N-TES

Synthetic DNA

pCMV-Sport6

ATGCGGTGACCTAGCTGCCGCGGCGGT

GCATGCGATCGCTTGCTGTCTCTGGAAGCA

L + A22-Q313 + AIA + H6

His6

pCri9a-KL23

KL23

pS6-RECK∆C

pCri9a

ATGCCCATGGTAACGACTTTTGATAA

GCATCTCGAGGCTGTGCTCTGAGAGG

V + T697-S797 + LE + H6

His6

pCri9a-KL123

KL123

pS6-RECK∆C

pCri9a

ATGCCCATGGTATCAGAAGATGACCG

GCATCTCGAGGCTGTGCTCTGAGAGG

V + S621-S797 + LE + H6

His6

pET3a-MT1∆C

MMP-14 CD

pET3a

—

—

M + S24-G284

None

  1. All constructs are for extracellular expression of the respective proteins.
  2. *Restriction-site sequences and overhangs for restriction-free cloning are underlined.
  3. **Peptide sequence of the expressed protein. Amino acids derived from the construct are in bold. See also Fig. 1.
  4. ***Tag fused to the carboxy-terminus.
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