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Figure 1

From: Efficient Biocatalytic Preparation of Rebaudioside KA: Highly Selective Glycosylation Coupled with UDPG Regeneration

Figure 1

Biosynthesis of rebaudioside KA from rubusoside. (a) Scheme of the glycosylation of rubusoside by OleD. (b) Cascade reaction to couple the glycosylation of target compound (black ellipse) with UDPG regeneration. (c) Schematic diagram for improvement of rebaudioside KA production in E.coli. Abbreviation: OleD, glycosyltransferase; SUS1, sucrose synthase; pyrF, orotidine-5′-phosphate decarboxylase; pgi, glucose-6-phosphate isomerase; ugd, UDPG dehydrogenase; UDPGA, uridine diphosphate glucuronic acid, TCA, tricarboxylic acid.

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