Figure 5 | Scientific Reports

Figure 5

From: Efficient Biocatalytic Preparation of Rebaudioside KA: Highly Selective Glycosylation Coupled with UDPG Regeneration

Figure 5

Construction of redirected carbon metabolic pathways for UDPG biosynthesis. (a) Engineering strategies for broadening the metabolic flux toward UDPG biosynthesis in E. coli C-1. Overexpressed genes are shown in red and deleted genes are shown in blue with italic, (Δ) represents gene knock-out. The glycolysis, pyrimidine metabolism and regeneration cycle are expressed by gray, blue, and green rounded rectangles, respectively. Abbreviations: F6P, fructose-6-phosphate; TCA, tricarboxylic acid; G6P, glucose-6-phosphate PRPP, 5-phosphoribosyl-1-pyrophosphate; UMP, uridine monophosphate; UDP, uridine diphosphate; UTP, uridine triphosphate; UDPG, uridine diphosphate glucose; UDPGA, uridine diphosphate glucuronic acid; KA, rebaudioside KA; Ru, rubusoside; Suc, sucrose; Fru, fructose; G1P, glucose-1-phosphate. (b) Comparison of rubusoside conversion by the engineered strains. **p < 0.01 and ***P < 0.001 indicate the statistical significances between different groups.

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