Figure 2
Effect of mycoplasma infection on HC11 cell growth, morphology and gene expression levels. (A) Genomic DNAs were amplified with primers targeting the 16 S rDNA of mycoplasma. PCR products were separated by electrophoresis through 2% agarose. The image shows that mycoplasma DNA was amplified from M. arginini-infected and reinfected cells, but not from the cured, mycoplasma-free cells. The image is compiled from two gels, which are shown in their entirety the Supplemental materials, (B) HC11 cells that were infected or reinfected with M. arginini, or uninfected (cured) were seeded on 10 cm plates. Pictures were taken 48 h after seeding. (C) HC11 cells, seeded in 24 well plates, were counted after different time periods using a coulter counter. Shown are the means ± standard deviations (SD) from duplicate wells at each time point from a representative experiment. (D,E) The expression levels of Lcn2, IL-6 and TNFα and cyclophilin in HC11 cells were quantified by RT-qPCR. The relative mRNA levels in all samples were normalized to those in uninfected (cured) cells. M. arg: M. arginini-infected cells cured: mycoplasma-free cells, reinfected: cells reinfected with M. arginini. M. ferment: M. fermentans infected cells.
