Figure 6
Anxiety- and despair-like behaviors in females, and TPH2 immunoreactivity in males and females. (A) Time spent in the light compartment of the dark-light box by control and mutant females at the proestrous/estrous (PE-E) or diestrous (D1-D2) stage (n = 18–29 females per genotype). Two-way ANOVA shows an effect of cycle (ap < 0.01); posthoc analyses indicate a reduced time spent in the light compartment for mutant females at the P-E stage (p < 0.01 versus controls at the same stage). (B) Time spent in immobility in the forced swim test for females at the P-E or D1-D2) stage. Two-way ANOVA shows an effect of cycle (ap < 0.05); posthoc analyses indicate an increased time spent in immobility for mutant females at the P-E stage (p < 0.01 versus controls at the same stage). (C) Time spent swimming in the forced swim test for females at the P-E or D1-D2) stage. Two-way ANOVA shows an effect of cycle (ap < 0.05); posthoc analyses indicate a reduced time spent in swimming for mutant females at the P-E stage (p < 0.01 versus controls at the same stage). (D,E) Number of TPH2 neurons counted in the dorsal raphe of females (D) and males (E). Data are means ± S.E.M. of 6–8 animals per sex and per genotype; **p < 0.01 versus controls.
