Figure 2

Sample processing and analysis workflow. (a) Schematic showing the parts that composed the holder where the membrane is placed. (b) A blood sample is automatically processed through the microfiltration device, followed by immunostaining steps. The membrane is mounted on a microscope slide and images are acquired using the integrated imaging system (c). (d) Fluorescent events are automatically classified and counted by a machine-vision algorithm implemented in the imaging system. Scale bar: 1 mm.