Table 2 List of top hits of IP-MS/MS from RanBP9-TT MEFs following ranking by abundance.

From: Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin

RANK

UNIPROT ID

PROTEIN NAME

Note (Ref)

Protein abundance

Protein abundance

Protein abundance

BP9TT

WT

WT cross-linked

1

P69566

RanB9

CTLH

25

0

0

2

O89050

Mkln1

CTLH

17

0

1

3

Q8C6G8

Wdr26

CTLH

10

0

0

4

Q9D7M1

Gid8

CTLH

10

0

0

5

Q9DBR3

Armc8

CTLH

10

0

0

6

P22777

Pai1

 

8

0

0

7

Q4VC33

Maea

CTLH

8

0

0

8

Q80YX1

Tena

 

7

0

0

9

Q6VN19

RBP10

CTLH

4

0

0

10

Q80YQ8

Rmd5A

CTLH

4

0

0

11

P21981

Tgm2

 

4

0

0

12

Q9CPY6

Gid4

CTLH

4

0

0

13

P62700

Ypel5

CTLH

3

0

0

14

Q61001

Lama5

 

2

0

0

15

Q99K41

Emil1

 

2

0

0

16

Q9R118

Htra1

 

2

0

0

17

Q91YQ7

Rmd5B

CTLH

2

0

0

18

P04104

K2C1

 

1

0

0

19

P62274

RS29

 

1

0

0

20

O08638

Myh11

 

1

0

0

21

P01786

Hvm17

 

1

0

0

22

Q6PGN1

ERFE

 

1

0

0

23

Q8CIH5

Plcg2

 

1

0

0

24

Q64339

Isg15

 

1

0

0

25

Q9JIY5

Htra2

(*)

1

0

0

26

O08807

Prdx4

 

1

0

0

  1. With the exception of Mkln1 (known CTLH member), only protein hits that did not show any counts both in the non-cross-linked or cross-linked RanBP9 WT MEFs are listed. Protein ID, name, and number of PSMs of immunoprecipitated proteins from RanBP9-TT and WT MEFs are reported. CTLH = known member of the CTLH complex. (*) Mouse homolog of HTRA2 protein found in the 2018 study by Lampert et al. as potential interactor of both RMND5A and ARMC8.