Figure 7
Effect of TCDD on the growth and metastasis of LLC-derived tumors in female mice. Female C57BL/6 mice were fed ω3-rich diet 29, provided with or without TPPU, and either treated or untreated with TCDD. (a) Tumor volume was assessed after injection with LLC cells (n = 14 to 21/group). (b) Lung weights were determined approximately five weeks after resection of tumors of approximately 3,700 mm3 in size (see Supplemental Table S4d). The data in a and b are each derived from two independent experiments. (c) Female mice were fed with ω6-rich diet 28, and tumor growth was determined (n = 9 to 15 per group). (d) Lung weight was measured after resection of tumors approximately 2,400 mm3 in size. (See Supplementary Table S4e.) The data in c and d are each derived from two independent experiments. *p < 0.05, **p < 0.01, *p < 0.001 compared with the corresponding –TCDD groups. (e) The levels of EPHX2 mRNA were evaluated in normal lung tissue from female and male mice (n = 4/group) fed omega 3-rich diet 29 by RT-PCR (mean ± SEM, ***p = 0.001). (f). EPHX2 protein was detected in the same mice by immunocytochemistry.
