Figure 3 | Scientific Reports

Figure 3

From: Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection

Figure 3

Analysis of mice inoculated with recombinant WNV. C57BL/6 mice were inoculated intraperitoneally with 10,000 pfu EgCME-WT or EgCME-E-I159V (A and B), intracranially with 10 pfu NY-EgCME or NY-EgCME-E159V (C and D), or intracranially with 1 pfu NY-WT or NY-E-V159I (E and F). (A) Kaplan-Meier survival curves of mice inoculated intraperitoneally with EgCME-WT or EgCME-E-I159V (n = 10). n.s., not significant. (B) Brains were collected at 7 dpi and the viral titers were measured (n = 13). Statistical significance was assessed by the two-tailed Student’s t-test. n.s., not significant. (C) Kaplan-Meier survival curves of mice inoculated intracranially with EgCME-WT or EgCME-E-I159V (n = 15). **p < 0.01. (D) Brains were collected at 1, 3, and 6 dpi and the viral titers were measured. Statistical significance was assessed by the two-tailed Student’s t-test. *p < 0.05. (E) Kaplan-Meier survival curves of mice inoculated intracranially with NY-WT or NY-159I (n = 20). n.s., not significant. (F) The brains were collected at 3 or 6 dpi and the viral titers were measured. Statistical significance was assessed by the two-tailed Student’s t-test. n.s., not significant.

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