Table 5 Primer sequences for microsatellite analysis.
Locus | Mult. PCR | Dye | F-primer sequences (5′-3′) | Primer conc. | Ta | References |
|---|---|---|---|---|---|---|
R-primer sequences (5′-3′) | ||||||
PLO3–71 | A | PET | GCTTCCTTTAATTTTAACTAATTG§ | 0.35 | 56 | |
CAATCCTGTATCAGGTTTCC | ||||||
PLO-M20 | A | VIC | GATTCTTATGTCTCTTGGGA | 0.15 | 56 | |
AAGTGCTTCAAGAGAAGTGC§ | ||||||
PLO-M3 | A | NED | CTCCCATCTTCCTCTTTTCG | 0.1 | 56 | |
GTTGACAATTGCAGGACCAC | ||||||
PLO2–14 | A | FAM | AAGAGCGTAATAAAAGCTTAC | 0.35 | 56 | |
CAAATAACAAGTTTCAATTTGG | ||||||
PLO-M2 | A | PET | GGAAAACCACAGAGAGACGG | 0.3 | 56 | |
CTTGGCACAGAGCAGAATCC | ||||||
PFL11 | B | PET | CATGCAAATAACACGCAC | 0.4 | 52 | |
CTGAACAAGGTAGGAAAGTCACTC | ||||||
P140 | B | FAM | ACCAGGCAATGGTAATACAG | 0.15 | 52 | |
CCAGGAGGACTTGTCAGAT | ||||||
PFL9 | B | VIC | GCCTTCATTTAGTTGAGGTCAG | 0.2 | 52 | |
GCATTCTGTCAGTGGCTTTCAC | ||||||
PLO-M17 | B | NED | CTGCTGAGTAAGGAGTAAGG | 0.35 | 52 | |
TCCCCTGTACATATTCAGGC | ||||||
PLO3–86 | B | FAM | GATTGATAGATTAATTGGTCTTAACTTCC | 0.27 | 52 | |
CTGGATTATAAATCTGGCAAGAGCC |
