Figure 5
From: Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome
A BioID interaction screen identifies interactors for MACROD1 and TARG1. (a) HeLa cells seeded on glass coverslips were transfected with plasmids expressing C-terminally BirA-R118G-fused MACROD1, MACROD2 or TARG1, treated with 150 µM biotin overnight and fixed using 3.7% PFA. The cells were stained using streptavidin-AlexaFluor488 or with an HA-selective antibody and analysed using confocal microscopy. (b) HeLa cells were transfected as in (a), treated with 50 µM biotin overnight and lysed in RIPA buffer. Lysates were analysed using Western Blotting and streptavidin-HRP or an HA-selective antibody (whole blots are shown). (c) Gene ontology using PANTHER was performed with the proteins identified as interactor for MACROD1 and the enriched cellular components, molecular functions and molecular processes are displayed. Scale bars represent 10 µM. FDR = false discovery rate.
