Figure 6
From: Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome
The TARG1 interactome is highly enriched for RNA metabolic processes in diverse cellular compartments. (a) A treemap plot was generated with the Gene Ontology terms from the TARG1 BioID analysis highlighting enriched biological processes. The visualization of Gene Ontology functional enrichment for biological processes was made using REVIGO (http://revigo.irb.hr/). A list of the GO-terms is available as Supplementary Table S4. (b) List of the most significant cellular component terms associated with the interactors in the TARG1 BioID analysis obtained using the PANTHER toolkit. (c) HeLa cells were transfected with plasmids encoding N-GFP-TARG1 and RFP-TIA1 or RFP-Dcp1a, the latter two as markers of stress granules and P-bodies, respectively, and analysed using live-cell confocal microscopy. Scale bars represent 10 µM. BF = brightfield; FDR = false discovery rate; GO = gene ontology; RFP = red fluorescent protein.
