Figure 6 | Scientific Reports

Figure 6

From: Human microbiota-transplanted C57BL/6 mice and offspring display reduced establishment of key bacteria and reduced immune stimulation compared to mouse microbiota-transplantation

Figure 6

Relative quantification of genes measured in ileum of germ-free and mouse microbiota-colonized B6 and SW mice. n = 10♀, 10♂ (MM B6); 10♀,8♂ (MM SW); 4♀, 4♂ (GF B6) and 3♀, 4♂ (GF B6) (see Table 1). Genes encoding markers for cytotoxic T cells, T helper cells, regulatory T cells and dendritic cells (Cd4, Cd8a, Foxp3, Itgax), toll-like receptors, REG3ɣ and IL-17a (Tlr2, Tlr4, Reg3g, Il17a), B cells (Cd19, Cd22), and mucins and tight junction complex proteins occludin and tight junction protein 1 (Muc1, Muc2, Ocln, Tjp1) were measured in ileum of F1 SW and B6 mice with a B6 MM at 18 wk of age, and in GF B6 and SW mice at 6 wk of age. ANOVA with Tukey’s multiple comparisons post hoc test performed separately for males and females. GF = germ-free, MM = mouse microbiota, B6 = C57BL/6NTac, SW = Tac:SW (Swiss Webster) P = transplanted parent generation, F1 = offspring generation born with the microbiota, RQ = relative quantification.

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